We now test for several genetic markers that have been found to be strongly linked to the tobiano coat pattern. In 2002, researchers at the University of Kentucky, led by Dr. Samantha Brooks, located a SNP within the KIT gene that is closely linked to the Tobiano pattern. Since then, additional markers have been found within the KIT gene by researchers here at Animal Genetics that we also use to determine Tobiano zygosity. Most recently, researchers at the University of Kentucky discovered a large chromosomal inversion on chromosome 3 near the KIT gene of Tobiano horses. This inversion, which has only been found in Tobiano horses, is believed to disrupt the normal functioning of the KIT gene and cause the Tobiano pattern.